M519MS培养基M519 Murashige & Skoog Basal Medium with Vitamins
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- 品牌名:PhytoTechnology
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MS 培养基是1962 年由Murashige 和Skoog 为培养烟草细胞而设计的。特点是无机盐和离子浓度较高,为较稳定的平衡溶液,在配制、贮存、消毒等过程中,即使有些成分略有出入,也不致影响离子间的平衡。其养分的数量和比例较合适,可满足植物的营养和生理需要,广泛地用于植物的器官、花药、细胞和原生质体培养,效果良好,它的液体培养基用于细胞悬浮培养时能获得明显成功。是目前普遍使用的培养基,有些培养基是由它演变而来的。
配方明细表(g/L)
Ammonium Nitrate | 1650 |
Boric Acid | 6.2 |
Calcium Chloride, Anhydrous | 332.2 |
Cobalt Chloride•6H2O | 0.025 |
Cupric Sulfate•5H2O | 0.025 |
Na2EDTA•2H2O | 37.26 |
Ferrous Sulfate•7H2O | 27.8 |
Magnesium Sulfate, Anhydrous | 180.7 |
Manganese Sulfate•H2O | 16.9 |
Molybdic Acid (Sodium Salt)• 2H2O | 0.25 |
Potassium Iodide | 0.83 |
Potassium Nitrate | 1900 |
Potassium Phosphate, Monobasic | 170 |
Zinc Sulfate•7H2O | 8.6 |
Glycine (Free Base) | 2 |
myo-Inositol | 100 |
Nicotinic Acid (Free Acid) | 0.5 |
Pyridoxine•HCI | 0.5 |
Thiamine•HCI | 0.1 |
Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working
Concentration: 4.43 g/L
Storage Temp of: 2-6°
Stock Solution:Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962).
pH = 3.5-4.5
说明书:http://www.phytotechlab.com/TechInfo/M519-info.pdf
MS培养基系列产品
货号 | 品名 | 中文名称 |
M519 | M & S BASAL MEDIUM w/VITAMINS | MS培养基 |
M524 | MURASHIGE & SKOOG BAS SALT MIX | MS培养基基础盐 |
M153 | MURASHIGE & SKOOG MOD SALT MIX | 1/2MS盐 |
M502 | M & S MACRONUTRIENT SALT BASE | MS大量元素 |
M654 | M & S MACRONUTRIENT STOCK SOL | MS大量元素储存液(10X) |
M554 | MS MICRONUTRIENT SALT BASE | MS微量元素(含铁盐) |
M529 | M & S MICRONUTRIENT STOCK SOL | MS微量元素溶液(含铁盐 10X) |
M533 | M & S VITAMIN POWDER (1000x) | MS维生素(1000X) |
M553 | M & S VITAMIN SOLUTION (1000x) | MS维生素溶液(1000X) |
M404 | M & S BASAL MEDIUM w/ GAMB VIT | MS盐与B5维生素混合物 |
M561 | MURASHIGE & SKOOG MOD SALT MIX | MS培养基(1/2硝酸盐) |
M290 | MURASHIGE & SKOOG MOD SALT MIX | MS培养基(含1/2硝酸盐与1/2钙盐) |
M531 | M & S MODIFIED BASAL SALT MIX | MS培养基含(不含氮) |
M407 | MURASHIGE & SKOOG MOD SALT MIX | MS培养基(不含氮磷钾) |
M571 | MURASHIGE & SKOOG MOD SALT MIX | MS培养基(不含硝酸铵) |
M5800 | MS BASAL MEDIUM w/FeNaEDTA | MS培养基(含FeNaEDTA) |
M530 | M & S MODIFIED BASAL MEDIUM | MS培养基(含MES) |
M5501 | MS MEDIUM w/VITAMINS & SUCROSE | MS培养基(含30g/L蔗糖) |
M5530 | MS MEDIUM MOD STOCK SOL with30 g/L Sucrose | MS培养基储存液(含30g/L蔗糖) |
M5615 | MS LIQUID MEDIUM w/ SUGARS | MS改良培养基(含15 g/L蔗糖和15 g/L葡萄糖) |
M5707 | MS MEDIUM w/VITAMINS & SUCROSE | MS改良培养基(含10 g/L蔗糖和20 g/L葡萄糖) |
M5825 | MS MED w/VIT, SUCROSE & GELZAN | MS改良培养基(含蔗糖、结冷胶) |
M499 | MURASHIGE & SKOOG MOD SALT MIX | MS改良培养基 (含FeNa-EDTA) |
M401 | M & S MODIFIED BASAL MEDIUM | MS改良培养基(含BA NAA 蔗糖) |
M535 | M & S MODIFIED BASAL MEDIUM | MS改良培养基(含硫酸腺嘌呤) |
M702 | M & S MODIFIED BASAL MEDIUM | MS改良培养基(含蔗糖和2ip) |
M516 | M & S BC POTATO MEDIUM | MS马铃薯培养基 |